Molecular and adaptive evolution of Nep2 gene from carnivorous plant Nepenthes

Alamsyah, Firman and Motomi, Ito (2020) Molecular and adaptive evolution of Nep2 gene from carnivorous plant Nepenthes. IOP Conference Series: Earth and Environmental Science, 457 (1). 012012. ISSN 1755-1315

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Nepenthes get their nutrient by carnivory using their pitchers. A prey drowned in the pitcher fluid, will be digested by enzymes called nepenthesin, i.e. nepenthesin II. The structure of nepenthesin II-encoding gene might be related to the role of the enzyme. Therefore, the objective of this study was to examine the molecular and adaptive evolutions of Nep2 gene expressing nepenthesin II. We analyzed 29 Nepenthes species that represent most habitat types. Total DNA was extracted from silica-dried leaf samples and amplification of Nep2 gene was performed using degenerate primers. Homology searching was conducted using BLASTn, followed by computation of isoelectric point of the enzyme, and testing for positive selection using Mega 5. The result showed 29 DNA sequences of Nep2 gene have no introns. Intron-less Nep2 gene will produce nepenthesin II rapidly to digest the prey. The gene experienced significant positive selection on N. sumatrana, a species inhabits the lowest altitude habitats amongst Sumatran species. An obvious adaptive phenotype is the development of two unusual types of lower pitchers to obtain nutrient in lowland habitats. In conclusion, molecular and adaptive evolutions of Nep2 gene characterized Nepenthes as highly adaptable plants that actively respond to the environmental stress.

Item Type: Article
Additional Information: This article is indexed in Q3 Journal Ranking Index, after presented in The 3rd International Conference on Biosciences held on 8 August 2019 at IPB International Convention Centre, Bogor - Indonesia; TURNITIN's similarity index result was checked based on rules stated in "Pedoman Operasional Penilaian Angka Kredit Kenaikan Jabatan Akademik/Pangkat Dosen" ( pp. 23-25
Uncontrolled Keywords: Nepenthes, Nep2 gene, molecular and adaptive evolution
Subjects: 500 Natural sciences & mathematics > 500 Science
500 Natural sciences & mathematics > 580 Plants (Botany)
Divisions: Universitas Al-Azhar Indonesia (UAI) > Fakultas Sains dan Teknologi (FST) > Biologi
Depositing User: Rifda Jilan
Date Deposited: 22 Sep 2021 02:26
Last Modified: 22 Nov 2021 10:52

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