DNA barcodes of the pleco (loricariidae, pterygoplichthys) in the Ciliwung River

Rosnaeni and Elfidasari, Dewi and Fahmi, Melta Rini (2017) DNA barcodes of the pleco (loricariidae, pterygoplichthys) in the Ciliwung River. International Journal of Advanced Research, 5 (2). pp. 33-45. ISSN 2320-5407

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Official URL: http://dx.doi.org/10.21474/IJAR01/3113


Pleco (Pterygoplichthys sp.) is an invasive freshwater species belong to LoricariidaeFamily and comes from Costa Rica, Panama, South America. Distribution of pleco spread throughout the freshwater of the world. One of the habitats in Indonesia is the Ciliwungriver and along the river with high abundance. Until now there has been no research results related to molecular identification of the fish especially from Ciliwung river. Therefore, it is necessary to know the molecular analysis of DNA to identify pleco from Ciliwungriver by using the technique of DNA barcoding gene CO1. The methodology consisted samples preparation, DNA extraction, DNA quantification, gene CO1 amplification, and DNA base sequence reading. Results identification using gene CO1 barcodes with a length of 650bp fragment showed that pleco from Ciliwung river has a 100% similarity with Pterygoplichthyspardalisspecies.There are transversion substitution nucleotide at nucleotide in positions 306 (C?T), 339 (G?A), 387 (C?T), and 471 (T?C), but this should not affect the amino acid sequence changes in pleco from Ciliwungriver.

Item Type: Article
Uncontrolled Keywords: Pleco, Ciliwung, Pterygoplichthys sp.
Subjects: 500 Natural sciences & mathematics > 570 Life sciences (Biology)
500 Natural sciences & mathematics > 590 Animals (Zoology)
Divisions: Universitas Al-Azhar Indonesia (UAI) > Fakultas Sains dan Teknologi (FST) > Biologi
Depositing User: Rifda Jilan
Date Deposited: 24 Aug 2020 07:18
Last Modified: 30 Sep 2020 16:55
URI: http://eprints.uai.ac.id/id/eprint/1395

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