DNA barcodes of the pleco (loricariidae, pterygoplichthys) in the Ciliwung River

Rosnaeni and Elfidasari, Dewi and Fahmi, Melta Rini (2017) DNA barcodes of the pleco (loricariidae, pterygoplichthys) in the Ciliwung River. International Journal of Advanced Research, 5 (2). pp. 33-45. ISSN 2320-5407

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Abstract

Pleco (Pterygoplichthys sp.) is an invasive freshwater species belong to LoricariidaeFamily and comes from Costa Rica, Panama, South America. Distribution of pleco spread throughout the freshwater of the world. One of the habitats in Indonesia is the Ciliwungriver and along the river with high abundance. Until now there has been no research results related to molecular identification of the fish especially from Ciliwung river. Therefore, it is necessary to know the molecular analysis of DNA to identify pleco from Ciliwungriver by using the technique of DNA barcoding gene CO1. The methodology consisted samples preparation, DNA extraction, DNA quantification, gene CO1 amplification, and DNA base sequence reading. Results identification using gene CO1 barcodes with a length of 650bp fragment showed that pleco from Ciliwung river has a 100% similarity with Pterygoplichthyspardalisspecies.There are transversion substitution nucleotide at nucleotide in positions 306 (C?T), 339 (G?A), 387 (C?T), and 471 (T?C), but this should not affect the amino acid sequence changes in pleco from Ciliwungriver.

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