Antiproliferative effect of electric fields on breast tumor cells in vitro and in vivo

Alamsyah, Firman and Ajrina, Izzatun Niswah and Dewi, Fitriya Nur Annisa and Iskandriati, Diah and Prabandari, Silvia Arin and Taruno, Warsito Purwo (2015) Antiproliferative effect of electric fields on breast tumor cells in vitro and in vivo. Indonesian Journal of Cancer Chemoprevention, 6 (3). pp. 71-77. ISSN 2355-8989

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Our research focused on the antiproliferative effect of low intensity (18 Vpp) and intermediate frequency (100 KHz) electrostatic wave between two capacitive electrodes on breast tumor cells in vitro and in vivo. In vitro study has been conducted by using MCF-7 cell lines treated with external electrostatic for 24, 48, and 72 hours of treatment and the cells number were calculated during treatment by using hemocytometer and presented as Growth Inhibition (GI)% efficacy. For in vivo, we used female mice (Mus musculus) strain C3H as animal model. The mice were injected with either MCF-7 cells, mammary tumor cells from C3H donor, or NaCl 0.9% (placebo) subcutaneously into the axilla area and exposed by external electrostatic in each cage for 12 hours in 2 weeks before necropsied. The adjacent and breast tissue were collected and stained with Hematoxylin – Eosin then analyzed for histopathological profile. In vitro study revealed the number of exposed cells decreased with lower proliferation rate than the non-exposed cells. Moreover, the external electrostatic caused 28-39% growth inhibition efficacy of MCF-7 cells. After 2 weeks of exposure, placebo mice were physically normal, whereas the tumor undergone significant shrinkage of more than 67% in size. Histopathological analysis of the mammary glands indicated infiltration of macrophages into the tumor area through the blood vessel. No abnormality was found in the skin layer and mammary glands of the breast tissue of placebo mice. Here, we present new knowledge of electro-capacitive cancer therapy (ECCT) as a novel treatment modality.

Item Type: Article
Additional Information: TURNITIN's similarity index result was checked based on rules stated in "Pedoman Operasional Penilaian Angka Kredit Kenaikan Jabatan Akademik/Pangkat Dosen" ( pp. 23-25
Uncontrolled Keywords: electro-capacitive cancer therapy (ECCT), tumor, in vitro, in vivo, breast cancer cells, antiproliferative
Subjects: 500 Natural sciences & mathematics > 500 Science
500 Natural sciences & mathematics > 570 Life sciences (Biology)
600 Applied sciences & technology > 610 Medicine & health
600 Applied sciences & technology > 610 Medicine & health > 616 Diseases
600 Applied sciences & technology > 610 Medicine & health > 619 Experimental Medicine
Divisions: Universitas Al-Azhar Indonesia (UAI) > Fakultas Sains dan Teknologi (FST) > Biologi
Depositing User: Rifda Jilan
Date Deposited: 22 Sep 2021 03:18
Last Modified: 22 Nov 2021 10:52

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