Effectiveness prebiotic and synbıotıc from composıte flour: Canna indica and Spirulina platensis

Noriko, Nita and Primasari, Alina and Istisqo, Sheila Syaifiyah (2019) Effectiveness prebiotic and synbıotıc from composıte flour: Canna indica and Spirulina platensis. In: The 2nd International Eurasian Conference on Biological and Chemical Sciences, 28-29 June 2019, Ankara, Turkey.

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Abstract

Prebiotic can stimulate growth of non pathogenic microflora in intestinal such as Bifidiobacteria and Lactobacillus with the result that prevent benefit of health. Sources of prebiotic that contain oligosaccharide were Canna indica and Spirulina platensis. Information about the effectiveness of C. indica and S. platensis as prebiotic and component of synbiotic based on in vivo treatment were minimal. Aims of the research were to know the effect of flour composite from C. indica and S. platensis (Cannalina) as prebiotic and synbiotic on ability to suppress total of microbe and support the growth and hold Lactobacillus (LAB) in intestine male Sprague dawley . The methodology was experimental design which divided for 4 groups for treatment. There were control, prebiotic, probiotic and sinbiotic groups. The division of control group which was mice fed up standard ration and 1 ml NaCl physiology. The prebiotic groups fed up pellet from Cannalina flour and 1 NaCl physiology, probiotic groups fed up pellet standard and LAB cultured 6.5 x 108 log cfu / ml in 1 ml NaCl and synbiotic group fed up Cannalina formed as the pellets and LAB cultured 6.5 x 108 log cfu/ml in 1 ml NaCl. The number of S. dawley in each groups were six mice. Bacteria from mice feces were isolated and analyzed to find the total of microbes and number of LAB. The results from in vivo treatment showed the highest total of microbe was found in control. probiotic, prebiotic and syntiotic have ability stimulated growth and hold of LAB in intestine compared with control in 10 days treatment and 5 days after treatment.. On 5 days after treatment synbiotic group was highest hold log 10,59 cfu/ml of LAB, and probiotic group was the lowest total of mecrobe log 9,21 cfu/ml.

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