Karina and Samudra, Muhammad F. and Rosadi, Imam and Afini, Irsyah and Widyastuti, Tias and Sobariah, Siti and Remelia, Melinda and Puspitasari, Riris Lindiawati and Rosliana, Iis and Tunggadewi, Tri I. (2019) Combination of the stromal vascular fraction and platelet-rich plasma accelerates the wound healing process: pre-clinical study in a Sprague-Dawley rat model. Stem Cell Investig, 6 (7). pp. 1-8. ISSN 2313-0792
Text (Article's Content)
ILS0202-23_Isi-Artikel.pdf - Published Version Download (1MB) |
|
Text (Plagiarism Check)
ILS0202-23_Cek-Turnitin.pdf - Supplemental Material Download (3MB) |
Abstract
Adipose-derived stem cells (ADSCs) improve wound healing owing to their angiogenic potency. However, the production of large quantities of ADSCs for clinical applications is laborious. In this study, the efficacy of the stromal vascular fraction (SVF; non-expanded ADSCs) combined with platelet-rich plasma (PRP) which contains abundant growth factors, for wound healing was evaluated using an animal model. PRP from venous blood and SVF from lipoaspirates were harvested from six donors. PRP, SVF, SVF + PRP, and saline solution as a negative control were injected to second degree burn wounds in the backs of 24 male Sprague-Dawley rats. On the seventh day after injection, rats were euthanized and wounds were analyzed microscopically and macroscopically. Wounds closed faster in the SVF + PRP group than in the control group or PRP or SVF alone groups, with less inflammation, prominent signs of re-epithelization, more skin appendages and blood vessels, and a higher rate of hair growth. No infection or rat death occurred during the trial. The combination of SVF and PRP may provide an additive stimulatory effect to support angiogenesis and accelerate the wound healing process; accordingly, this combination is a potential alternative to ADSC treatment.
Item Type: | Article |
---|---|
Additional Information: | TURNITIN's similarity index result was checked based on rules stated in "Pedoman Operasional Penilaian Angka Kredit Kenaikan Jabatan Akademik/Pangkat Dosen" (http://lldikti12.ristekdikti.go.id/wp-content/uploads/2019/03/PO-PAK-2019_MULAI-BERLAKU-APRIL-2019.pdf) pp. 23-25 |
Uncontrolled Keywords: | Angiogenesis; platelet-rich plasma (PRP); stromal vascular fraction (SVF); wound healing |
Subjects: | 500 Natural sciences & mathematics > 570 Life sciences (Biology) |
Divisions: | Universitas Al-Azhar Indonesia (UAI) > Fakultas Sains dan Teknologi (FST) > Biologi |
Depositing User: | Rifda Jilan |
Date Deposited: | 29 Apr 2023 02:20 |
Last Modified: | 29 Apr 2023 02:25 |
URI: | http://eprints.uai.ac.id/id/eprint/2264 |
Actions (login required)
View Item |